It can be applied to multiple areas of basic research in medicine and biology, such as early clinical diagnosis of diseases, pathogen detection, tumor-related genes and drug resistance genes detection, gene expression levels in tissues or cells, transgenic animal and plant testing.
Technology advantage
Monitor and display fluorescence changes in real time;
Equipped with an excellent temperature control system to ensure accurate, uniform temperature control and rapid ramp rate;
Simultaneous detection of 4/6 fluorescence;
Suitable for a variety of scientific and clinical reagents;
Suitable fordye-based assays and Taqman assays;
Suitable for common PCR tubes, eight-row tubes, 96-well plates, etc. , 0.1ml volume is recommended.
Sample capacity | 0.2ml PCR tubes×96, 8×12 PCR plate or 96 well plate ×1 |
Reaction volume | 10-50 μl |
Thermal cycle technology | Peltier |
Max. Heating/Cooling rate | 6.0° C/s |
Heating temperature range | 4 – 100 °C |
Temperature accuracy | ± 0.2°C |
Temperature uniformity | ±0.2℃ @60℃ , ±0.2℃ @95℃ |
Temperature gradient setting range | 30–100°C |
Temperature gradient difference setting range | 1 – 36 °C |
Detection system | |
Excitation light source | 4* monochrome high efficiency LEDs |
Detection device | PMT |
Detection mode | Time-resolved signal separating technology |
Excitation/detection wavelength range | 455-650nm/510-715nm |
Fluorescent channels | 4 channels |
Supported dye | FAM/SYBRGreen, |
VIC/JOE/HEX/TET, | |
ROX/TexasRed, | |
Cy5®/Quasar® 670 | |
Sensitivity | Single copy gene |
Resolution | 1.33 folds copy number difference can be distinguished in single-plex qPCR |
Dynamic range | 10 orders of magnitude copies |
Analysis mode | |
Absolute quantification, Melting curve analysis, Endpoint fluorescence analysis | |
Data output | |
Original file, Excel data file, results include run settings, graph and tabular data results | |
