Metronidazole sodium chloride injection is a kind of preparation used to treat anaerobic infection, almost colorless and transparent. The active ingredient is metronidazole, and the auxiliary materials are sodium chloride and water for injection. Metronidazole is a nitroimidazole derivative, which is prone to appear the degradation product nitrite after sterilization. Nitrite can oxidize the normal oxygen carrying low iron hemoglobin in the blood into methemoglobin, which will lose its oxygen carrying capacity and cause tissue hypoxia. If the human body ingests too much nitrite in a short time, it may cause poisoning, and in serious cases, it may also lead to cell canceration. Therefore, it is necessary to determine the nitrite content in metronidazole sodium chloride injection.

Instruments and equipment 

CIC-D120 Ion chromatograph,SHRF-10 Eluent generator and IonPac AS18 column

Sample chromatogram